Rabbit Human Copeptin Antibodies

This is an antibody preparation generated in rabbit by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel School of Medicine at Dartmouth College) using a synthetic peptide representing the last 18 C-terminal residues of the 39 amino acid glycopeptide, coupled to bovine thyroglobulin as antigen. 

Specificity: No measureable reactivity with rat or mouse copeptin, no measureable reactivity with vasopressin or human vasopressin-related neurophysin. 

Tested applications: IHC-formaldehyde-fixed tissues, Flow Cytometry, RIA, ELISA 

Properties 

Form:                Liquid 

Storage Instructions: Shipped at 4o C. Store at 4o C short term (1-2 weeks). Upon delivery aliquot and store at -20o C or -80o C. Avoid freeze/Thaw cycling. 

Storage buffer: Preservative none.  Stable if stored as 1:100 dilutions in PBS containing 1.25 mg/ml of BSA at -20o C. Constituents: Whole serum 

Purity:              Whole Antiserum 

Research Areas: Neuroscience, Endocrinology, Neuropeptides, Hormones, Neuroendocrinology, Measurement of Oxytocin Release. 

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Woomera Therapeutics Inc.  guarantees use of BORIS Y in each of the tested applications: 

The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user. 

Application 

IHC:                  1/1000-1/2000 Use at an assay dependent concentration 

RIA:                        1/15,000-20,000, Sensitivity to 2 pg; sensitivity  range, 2-320 pg; 50% displacement at 40 pg; <no measurable cross-reaction with AVP or VP-HNP. 

Flow Cytometry: >1/1000 

Western:         >1/1000 

References: 

Friedmann, A.S., Malott, K.A., Memoli, V.A., Pai, S.I., Yu, X.-M., and North, W.G.  Products of vasopressin gene expression in small-cell carcinoma of the lung.  Br. J. Cancer  69:260-263, 1994. 

North, W.G., Pai, S., Friedmann, A., Yu, X., Fay, M., and Memoli, V.  Vasopressin gene related products are markers of human breast cancer.  Breast Cancer Research and Treatment 34:229-235, 1995.