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This antibody generated by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel School of Medicine at Dartmouth College) using vasopressin coupled to bovine thyroglobulin.
Specificity: Less than 0.1% reactivity with oxytocin in RIA Tested Applications IHC-formaldehyde fixed tissues, Flow cytometry , RIA, ELISA
Properties
Form: Liquid
Storage Instructions: Shipped at 4 o C. Store at 4 o C short term (1-2 weeks). Upon delivery aliquot and store at -20 o C or -80 o C. Avoid freeze/Thaw cycling.
Storage buffer: Preservative none. Stable if stored as 1:100 dilutions in PBS containing 1.25 mg/ml of BSA at -20 o C.
Constituents: Ascites at ~2mg/ml
Purity: Mouse Ascites
Clonality: Monoclonal
Isotype: IgG1
Research Areas: Neuroscience, Endocrinology, Neuropeptides, Hormones, Neuroendocrinology
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Woomera Therapeutics Inc. guarantees use of DEN1 in each of the tested applications:
The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user.
Application
IHC: Use at an assay dependent concentration; suggest~ 4 µg/ml (~1/500 Ascites)
RIA: Use at an assay dependent concentration
Western: >1/200 (~10 µg/ml)
Flow Cytometry/ICC: 1/100 to 1/200 (~10-20 µg/ml).
Product code: DEN
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