Rabbit Oxytocin-Rat/ Mouse Neurophysin Antibodies

This is an antibody preparation generated in rabbit by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel School of Medicine at Dartmouth College) using oxytocin-associated rat neurophysin (OT-RNP’,RNP3) protein purified by preparative electrophoresis as antigen.  

Specificity:  Less than 1% reactivity with Rat and Mouse Neurophysins 1(Vasopressin Associated Rat and Mouse Neurophysin), 100% cross-reaction with Mouse Oxytocin- Related Neurophysin (MNP3) and ~20-50% cross-reaction with OT-RNP (RNP2) and OT-MNP (MNP2).  

Tested Applications: IHC-formaldehyde fixed tissues, Flow cytometry , RIA, ELISA 

Properties 

Form: Liquid 

Storage Instructions: Shipped at 4o C. Store at 4o C short term (1-2 weeks). Upon delivery aliquot and store at -20o C or -80o C. Avoid freeze/Thaw cycling. 

Storage buffer:      Preservative none.  Stable if stored as 1:100 dilutions in PBS containing 1.25 mg/ml of BSA at -20o C. Constituents: Whole serum 

Purity:                      Whole Antiserum 

Clonality:                Polyclonal 

Isotype:                   IgG, Behaves chromatographically like mouse IgG2B 

Research Areas:     Neuroscience, Endocrinology, Neuropeptides, Hormones, Neuroendocrinology, Measurement of Vasopressin Release. 

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Woomera Therapeutics Inc.  guarantees use of PP in each of the tested applications: 

The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user. 

Application 

IHC:                  1/5000-1/10000, Use at an assay dependent concentration 

RIA:                  1/30,000; Sensitivity to 20 pg; range of sensitivity 20-1280 pg; 50% displacement at 80 pg; < 1% VP-RNP cross-reaction, 100% cross-reaction with OT-MNP’(MNP3); ~20-50% cross-reaction  with OT-RNP (RNP2) and OT-MNP (MNP2). 

ELISA: 1/10,000 

Flow Cytometry: 1/1000 

Western:         1/1000  

Reference 

North, W.G., and Valtin, H.  The purification of rat neurophysins by a method of preparative polyacrylamide gel electrophoresis.  Analyt. Biochem. 78:436-450, 1977. 

North, W.G., LaRochelle, F.T., Jr., and Hardy, G.R.  Radioimmunoassays for individual rat neurophysins.  J. Endocrinol. 96:373-386, 1983. 

North, W.G., and Mitchell, T.I.  Evolution of neurophysin proteins:  Partial amino acid sequences of rat neurophysins.  FEBS Letters 126:41-44, 1981. 

Fisher, A.W.F., Gill, V., Wong, K., Raghatvan, S., North, W., and Lederis, K.  Anatomical and developmental aspects of the neurons terminating in the posterior pituitary.  In: Neurosecretion: Molecules, Cells, Systems. D.S. Farner and K. Lederis (eds.) Plenum Publishing Corp. pp. 39-47, 1982. 

Wong, K.W., Fisher, A., Lederis, K., and North, W.  The ontogeny of the oxytocin/neurophysin system of the Brattleboro rat.  Ann. N.Y. Acad. Sci. 394:177-182, 1982. 

North, W.G., Gellai, M., and Hardy, G.  Oxytocin and oxytocin-associated neurophysin evaluation by RIA in the Brattleboro rat: Turnover.  Ann. N.Y. Acad. Sci. 394:167-172, 1982. 

Cheng, S.W.T., and North, W.G.  Effects of acute increases in plasma osmolality on plasma vasopressin-associated neurophysin in conscious rats: implications for osmoregulation.  Neuroendocrin. 40:363-368, 1985. 

Cheng, S.W.T., and North, W.G.  Function of vasopressinergic neurons in rats under conscious and anesthetized conditions.  Am. J. Physiol. 248:E155-E161, 1985. 

Stern, J.E., Mitchell, T., Herzberg, V.L., and North, W.G.  Secretion of vasopressin, oxytocin and two neurophysins from rat hypothalamo-neurohypophyseal explants in organ culture.  Neuroendocrin., 43:252-258, 1986. 

Gonzalez, C.B., Caorsi, C.E., North, W.G., and Berrios, O.T.  Purification of bovine neurosecretory granule membranes by density gradient centrifugation. Analytical Biochem. 157:316-322, 1986. 

O'Connor, E.F., Cheng, S.W.T., and North, W.G.  Effects of intraperitoneal injection of lithium chloride on neurohypophyseal activity: implications for behavioral studies.  Physiology and Behavior 40:91-95, 1987.  

Cheng, S.W.T., and North, W.G.  Reduced responsiveness to acute salt-loading of vasopressin-neurons and oxytocin-neurons in the heterozygous Brattleboro rat. Neuroendocrinol. 45:93-98, 1987. 

Cheng, S.W.T., and North, W.G.  Vasopressin reduces release from vasopressin-neurons and oxytocin-neurons by acting on V2-like receptors. Brain Res. 479:35-39, 1989. 

Cheng, S.W.T., O'Connor, E.F., and North, W.G.  Influence of opioid peptides on release from vasopressin and oxytocin neurones of the hypothalamoneurohypophyseal system:  Effects of naloxone in the conscious rat.  Can. J. Physiol. Pharmacol. 68:568-574, 1990. 

Cheng, S.W.T., and North, W.G.  Influence of pentobarbital and urethane on release from magnocellular neurons.  Neuroendocrinology  54:482-487, 1991. 

North, W.G., O'Connor, E.F., and Gonzalez, C.B.  Single-step isolation and sequencing of vasopressin and oxytocin precursors.  Peptides 13:395-400, 1992. Product Code: FD76