Rabbit Oxytocin Bovine (cow) Neurophysin Antibodies

These are antibody preparations generated in rabbit by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel School of Medicine at Dartmouth College) using oxytocin-associated bovine neurophysin protein purified by preparative electrophoresis as antigen. 

Specificity:  Less than 1% reactivity with Bovine Neurophysin2 (Vasopressin-Associated Bovine Neurophysin, VP-BNP) in RIA 

Tested Applications: IHC-formaldehyde fixed tissues, Flow cytometry , RIA, ELISA 

Properties 

Form: Liquid 

Storage Instructions: Shipped at 4o C. Store at 4o C short term (1-2 weeks). Upon delivery aliquot and store at -20o C or -80o C. Avoid freeze/Thaw cycling. 

Storage buffer:     Preservative none.  Stable if stored as 1:100 dilutions in PBS containing 1.25 mg/ml of BSA at -20o C. Constituents: Whole serum 

Purity:                         Whole Antiserum 

Clonality:                  Polyclonal 

Isotype:                          IgG, Behaves chromatographically like mouse IgG2B

Research Areas:          Neuroscience, Endocrinology, Neuropeptides, Hormones, Neuroendocrinology, Measurement of Oxytocin Release.   

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Woomera Therapeutics Inc.  guarantees use of FPY, CRS, DORGHT in each of the tested applications: 

The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user. 

Application 

IHC:                Use at an assay dependent concentration 

RIA:          1/20,000; Sensitivity to 20 pg; range of sensitivity 20-1280 pg; < 1% VP-BNP cross-reaction. 

Western :        >1/1000