VASOPRESSIN ANTIBODIES (FZE)

This is an antibody preparation generated in rabbit by Dr. William North Professor of Physiology and Neurobiology of Dartmouth Medical School (now Geisel Medical School at Dartmouth) using arginine- vasopressin coupled to bovine thyroglobulin. 

Specificity: Less than 0.1% cross-reactivity with oxytocin, in RIA 

Tested Applications IHC-formaldehyde fixed tissues, Flow cytometry, , RIA, ELISA 

Properties 

Form:                              Liquid 

Storage Instructions: Shipped at 4 o C. Store at 4 o C short term (1-2 weeks). Upon delivery aliquot and store at -20 o C or -80 o C. Avoid freeze/Thaw cycling. 

Storage buffer:           Preservative none -Stable if stored as 1:100 dilutions in PBS containing  1.25 mg/ml of BSA at -20 o C. 

Constituents:              Whole serum 

Purity:                           Whole Antiserum 

Clonality:                     Polyclonal 

Isotype:                       IgG, Behaves chromatographically like mouse IgG2B 

Research Areas:       Neuroscience, Endocrinology, Neuropeptides, Hormones,  Neuroendocrinology, OBGYN, Cancer  

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Woomera Therapeutics Inc. guarantees use of FZE in each of the tested applications: 

The application information recommends certain starting dilutions; however, optimal dilutions/concentrations should be determined by the end user. 

Application  

IHC:                           Use at an assay dependent concentration 

RIA:                            1/30,000-99,000. Sensitivity to 0.4 pg/ml; 50% displacement ~5 pg,  range 0.4-32 pg; <0.1% OT cross-reaction.  

ELISA:                         1/30,000-50,000 

Flow Cytometry:      1/1000-5000 

Western:                    1/1000-5000

References

North, W.G. Neuropeptide production by small-cell carcinoma: Vasopressin and oxytocin as plasma markers of disease. J. Clin. Endocrin. Metab. 73:1316-1320, 1991. 

Cheng, S.W.T., and North, W.G. Absence of negative feedback by oxytocin on release from magnocellular neurones in conscious rats. Can. J. Physiol. Pharmacol. 70(1):100-105, 1992.

Piqueux, C., Hagelstein M.T., Keegan, B., Legros, V., and North, W.G. Vasopressin and Oxytocin mitogen transduction pathway in small cell lung cancer cells. Endocr.-Relat. Cancer, 4:871- 875, 2004.  

Product Code FZE